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On June 15, 2018, following an investigation by the U. Attorney's Office in San Francisco that lasted more than two years, a federal grand jury indicted Holmes and former Theranos chief operating officer and president Ramesh "Sunny" Balwani on nine counts of wire fraud and two counts of conspiracy to commit wire fraud. Prosecutors allege that Holmes and Balwani engaged in two criminal schemes, one to defraud investors, the other to defraud doctors and patients.

In June 2019, Bloomberg News reported Holmes and Balwani are looking into a possible defense strategy of blaming the media for the downfall of Theranos.

On learning of the investigation, Holmes initiated through her lawyer David Boies a campaign to stop Carreyrou from publishing, by levying legal and financial threats against the Journal and the whistleblowers.

The lawyers are looking into whether journalist John Carreyrou's reporting caused undue influence upon government regulatory agencies in order to write a sensational story for The Wall Street Journal. Senator (D-GA); and former CEOs Dick Kovacevich of Wells Fargo and Riley Bechtel of Bechtel.

She helped to draft and pass a law in Arizona to let people obtain and pay for lab tests without requiring insurance or healthcare provider approval, while misrepresenting the accuracy and effectiveness of the Theranos device. Before being exposed and the subsequent collapse of Theranos, Holmes received widespread acclaim.

She appeared on Jim Cramer's Mad Money the same evening the article was published.

Cramer said, "The article was pretty brutal", to which Holmes responded, "This is what happens when you work to change things, first they think you're crazy, then they fight you, and then all of a sudden you change the world." In January 2016, the Centers for Medicare and Medicaid Services (CMS) sent a warning letter to Theranos after an inspection of its Newark, California laboratory uncovered irregularities with staff proficiency, procedures, and equipment. Department of Health and Human Services appeals board.

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